Spore germination Tek
Spore germination kit
Germinating your spores will give you much better results and speed up the process.
Step 1: Simply take your pre measured liquid culture base and inject it into your jar
Step 2: Add 250 ml of Non-chlorinated water (jar has a mark)
Step 3: Close lid and cover it with aluminum foil (to keep water off the filter patch)
Step 4: Sterilize the best method is to Pressure cook jar for about 45 min
Step 5: inject your spores
It is important to use a sterile method when injecting your spores. The simplest way is. First wipe injection port with alcohol wipe or paper towel soaked in alcohol. Wear gloves if needed and use a lighter to flame the tip of the needle till red. Then place the tip while red into a folded alcohol wipe or paper towel soaked in alcohol. This allows you to prepare to inject without risk of recontamination getting your needle tip right to your injection port. Pull needle out and inject it into the port. After about 5 days you will see a cloudy fuzz forming this is the mycelium growing. Some people spray lysol or a disinfectant but this is not a must.
It is important to swirl your jar during this time to keep the mycelium broken up. Repeat the sterile needle tek each time you inject a needle to withdraw mycelium to prevent contamination.
Without a pressure cooker it can be done using a microwave to boil your Liquid culture or a water bath canner but these methods may not be as effective as the above described.
Liquid culture tek
This is instructions for making a Liquid Culture solution.
500 ml of non-chlorinated uninstalled water
20 ml of light corn syrup
Pressure cook for 45 min to sterilize
You will need a pressure cooker and jars. The jars will have the 500 ml mark on the side but for the corn syrup you will need something to measure out 20 ml, a syringe works best. You will need to modify your lid to allow you to inject your mycelium or spores to germinate them.
We have injection ports for this.
Rye Grain Prep
Here are basic steps for preparing your rye grain to be inoculated.
Rinse your grain clean
Simply soak your rye grain for 8 to 12 hours in water add one tablespoon of gypsum.
Drain your rye and add to pot to boil for 5 to 10 min. Drain again and let cool this will help to evaporate the moisture add vermiculite to get moisture content right. The grain should not have any visible water and should not stick together and should pour right out of your hand.